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KMID : 0358219960230010109
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Korean Journal of Fertility and Sterility
1996 Volume.23 No. 1 p.109 ~ p.114
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Preimplantation Genetic Diagnosis Using Primer Extension Preamplification in Duchenne/Becker Muscular Dystrophy(DMD/BMD) Families
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최수경
이은호/이호준/전진현/강인수/백은찬/류현미/전종영
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Abstract
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General PCR technique alone has a limitation for preimplantation genetic diagnosis (PGD) using single blastomere. Recently developed primer extension preamplification (PEP) technology amplifies the whole genome and thus, simultaneous multiple
locus
analysis became possible. In this study, we report the efficacy of PEP-PCR for PGD in three muscular dystrophy carries undergoing IVF-ET. A total of 37 blastomeres were obtained from 40 embryos at six to eight cell stage in three IVF cycles in
two
DMD
and one BMD carries. Whole genome from single blastomeres were amplified using 15-base oligonucleotide random primers.
PCR amplified products of exon 45 in the dystrophin gene and alphoid X/Y loci for gender determination were analysed by 2% metaphor gel electrophoresis.
A total of 37 PEP-PCR replicates from 37 single blastomeres from 40 embryos and 37 blanks were performed. We obtained the reliable results for exon 45 and alphoid X/Y. Transfer of female embryos and unaffected male embryo was attempted in three
couples.
Unfortunately, pregnancy was not achieved in these cases. PEP-PCR is a reliable and efficient PGD method in multiple locus analysis using single blastomere.
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